Regulatory

Part:BBa_K563003

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)


promoter of CIT2

CIT2 encodes Citrate synthase. Its promoter is controlled by rtg1 transcription factor. When TORC1 is inactive, Rtg1 tranlocates into the nuclear and promoter the expression of CIT2.In our project, we will promoter our mut-Tor2 by the promoter of CIT2 and construct the feed back loop.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 475
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apromoter of CIT2